ERA

Download the full-sized PDF of Asialofetuin-coated PLGA Nanoparticles for Targeting HepatocytesDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R32K8C

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Asialofetuin-coated PLGA Nanoparticles for Targeting Hepatocytes Open Access

Descriptions

Other title
Subject/Keyword
asialofetuin
hepatocytes
nanoparticles
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Kharaud, Gagandeep
Supervisor and department
Kaur, Kamaljit (Faculty of Pharmacy and Pharmaceutical Sciences)
Examining committee member and department
Uludag, Hasan (Chemical and Materials Engineering, Faculty of Engineering)
Lavasanifar, Afsaneh (Faculty of Pharmacy and Pharmaceutical Sciences)
Suresh, Mavanur (Faculty of Pharmacy and Pharmaceutical Sciences)
Department
Faculty of Pharmacy and Pharmaceutical Sciences
Specialization

Date accepted
2010-08-31T22:18:58Z
Graduation date
2010-11
Degree
Masters of Science in Pharmaceutical Sciences
Degree level
Master's
Abstract
The purpose of this project was to formulate, modify and evaluate nanoparticles for targeting asialoglycoprotein receptor found in large numbers exclusively on hepatocyte surfaces. Our goal is to increase the efficacy, reduce the side effects and the cost of the hydrophilic drugs administered to cure diseases like hepatitis C virus. Selective drug delivery into targeted cells using nanoparticles is one effective approach to enhance activity and avoid systemic side effects. Here we describe our effort towards the development of specially engineered poly(D,L-lactic-co-glycolic acid) nanoparticles using double emulsion method and surface coat them with asialofetuin for targeted delivery into hepatocytes. Bovine Serum Albumin-coated nanoparticles were prepared as a negative control. Furthermore, covalently conjugated protein on nanoparticle was labeled with rhodamine and was used for cell-based studies. Results from these studies indicated that asialofetuin conjugated with nanoparticles showed enhanced and selective uptake by hepatocytes compared to nanoparticles conjugated with Bovine Serum Albumin.
Language
English
DOI
doi:10.7939/R32K8C
Rights
License granted by Gagandeep Kharaud (gkharaud@pharmacy.ualberta.ca) on 2010-08-31T22:09:08Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-05-01T04:12:25.214+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 3988610
Last modified: 2015:10:12 12:29:02-06:00
Filename: Kharaud_Gagandeep_Fall 2010.pdf
Original checksum: 9ca1f20c500952770f00e6d6b30aa325
Well formed: true
Valid: true
File title: Microsoft Word - Gagan Thesis_Final
File author: krish
Page count: 109
Activity of users you follow
User Activity Date