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Permanent link (DOI): https://doi.org/10.7939/R3FS8V

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Desiccated and Preserved Polyacrylamide based Nucleic Acid Diagnostic Systems Open Access

Descriptions

Other title
Subject/Keyword
Desiccation
Diagnostics
Nucleic Acid
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Chavali Venkata Subramanya, Ravi Shankar
Supervisor and department
Acker, Jason (Laboratory Medicine and Pathology)
Pilarski, Linda (Oncology)
Examining committee member and department
Acker, Jason (Laboratory Medicine and Pathology)
Lai, Raymond (Laboratory Medicine and Pathology)
Yanow, Stephanie (Provincial Laboratory for Public Health)
Pilarsk, Linda (Oncology)
Department
Medical Sciences- Laboratory Medicine and Pathology
Specialization

Date accepted
2013-01-30T11:23:49Z
Graduation date
2013-06
Degree
Master of Science
Degree level
Master's
Abstract
The In-gel polymerase chain reaction (PCR) technology developed by our group at the University of Alberta makes use of PCR reagents entrapped within nano-liter polyacrylamide gels. To adapt this technology for use at the point of care, methods were developed to preserve gel-based PCR reagents by desiccation within the microchips. The gel-based PCR reagents were desiccated using a vacuum desiccator with trehalose as the lyoprotectant. Long-term stability studies were conducted with these formulations at different temperature conditions. Our results indicate that the desiccated gel-based PCR reagents can retain most of their activity upon rehydration after ten weeks. The shelf life stability of these desiccated gel-based PCR reagents can be maintained for much longer durations with further improvements to the formulations, chip geometries and packaging strategies. With the inclusion of the desiccated gel-based PCR reagents within the chips, the in gel PCR technology has a great potential to be used at point of care.
Language
English
DOI
doi:10.7939/R3FS8V
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File format: pdf (Portable Document Format)
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File size: 6873331
Last modified: 2015:10:12 11:31:57-06:00
Filename: Chavali_Ravi_Spring 2013.pdf
Original checksum: 25fa34954e95cf4e276aff9fc6058488
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File title: Title page final
File title: Microsoft Word - Title page final.doc
File author: Ravi Chavali Venkata
Page count: 175
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