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Permanent link (DOI): https://doi.org/10.7939/R3K88X

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Dimerization of the DEAD-Box Cyanobacterial RNA Helicase Redox, CrhR Open Access

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Other title
Subject/Keyword
Dimerization
Helicase
DEAD-box
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Skeik, Reem M
Supervisor and department
Owttrim, George (Biological Sciences)
Examining committee member and department
Fahlman, Richard (Biochemistry)
Leskiw, Brenda (Biological Sciences)
Department
Department of Biological Sciences
Specialization
Molecular Biology and Genetics
Date accepted
2012-06-01T11:41:33Z
Graduation date
2012-11
Degree
Master of Science
Degree level
Master's
Abstract
The DEAD-box cyanobacterial RNA helicase redox, or CrhR, in Synechocystis sp. PCC 6803 is capable of unwinding dsRNA and in annealing ssRNA in a bidirectional ATP-dependent manner. This is a feature shared by only four other DEAD-box RNA helicases. Two of which, the eukaryotic p68 and p72 proteins, were also shown to self-dimerize. Self-dimerization is a characteristic rarely possessed by an RNA helicase. In this study, CrhR was found to exhibit self-interaction using the yeast two-hybrid system and differentially tagged-CrhR protein exchange (swap) analysis, with the dimerization domain localized to the N-terminus, and some assistance, or partial dimerization occurring through the C-terminus. FPLC analysis also revealed CrhR dimerization to occur in an RNA-independent manner. In addition to FPLC analysis, mass spectrometry also suggests CrhR interaction with protein complexes in vivo. These findings suggest physiological functions for CrhR association with ribosomes in multi-subunit complexes upon acclimatization of Synechocystis cells to low temperature.
Language
English
DOI
doi:10.7939/R3K88X
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File author: Reem Skeik
Page count: 185
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