Download the full-sized PDF
Permanent link (DOI): https://doi.org/10.7939/R35717R7B
This file is in the following communities:
|Biological Sciences, Department of|
This file is in the following collections:
|Journal Articles (Biological Sciences)|
Mitochondrial DNA variation and identification of bark weevils in the Pissodes strobi species group in western Canada (Coleoptera: Curculionidae) Open Access
- Author or creator
Langor, D. W.
Sperling, F. A. H.
- Additional contributors
- Type of item
- Journal Article (Published)
Abstract: Morphological, allozyme, and chromosomal characters and ecological traits have limited value for discriminating among four closely related Pissodes spp. known from western Canada. We amplified a 1585-bp segment of mitochondrial DNA (mtDNA), including half of the cytochrome oxidase I (COI) and all of the tRNA leucine and COII genes, using the polymerase chain reaction, and studied mtDNA variation within and among all four species of Pissodes with restriction enzymes. Twenty-four haplotypes were found among the 121 maternal lineages surveyed. Haplotype distributions suggest intermediate levels of gene flow for each species. Interspecific estimated sequence divergences ranged from 0 to 28.7%. Phylogenetic relationships among species were reconstructed using P. affinis Randall as an outgroup. Pissodes terminalis Hopping and P. nemorensis Germar were the most closely related species, and this clade was most closely related to P. strobi (Peck); P. schwarzi Hopkins branched off below these three. Restriction site variation is sufficient to discriminate unambiguously among most species. However, P, terminalis and P. nemorensis haplotypes were very similar, which may complicate discrimination between these two species, using mtDNA characters, where their ranges putatively overlap in Manitoba. A diagnostic protocol using three restriction enzymes, Eel I, Dra I, and HinfI, is recommended.
- Date created
- License information
- © 1995 Cambridge University Press on behalf of the Entomological Society of Canada. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
- Citation for previous publication
Langor, D. W., & Sperling, F. A. H. (1995). Mitochondrial DNA variation and identification of bark weevils in the Pissodes strobi species group in western Canada (Coleoptera: Curculionidae). Canadian Entomologist, 127(6), 895-911.
- Link to related item
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1471657
Last modified: 2015:10:12 16:37:49-06:00
Original checksum: ab9c0be9b6ea507e02db1f49a7ca3f97
Well formed: true
Page count: 17