Download the full-sized PDF of Paracrine Effect of Mesenchymal Stromal Cells on Multifactorial Lung Injury in Neonatal MiceDownload the full-sized PDF



Permanent link (DOI):


Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Graduate Studies and Research, Faculty of


This file is in the following collections:

Theses and Dissertations

Paracrine Effect of Mesenchymal Stromal Cells on Multifactorial Lung Injury in Neonatal Mice Open Access


Other title
Conditioned Media
Mesenchymal Stromal Cells
Lung Injury
Bronchopulmonary Dysplasia
Type of item
Degree grantor
University of Alberta
Author or creator
Strueby, Lannae L.
Supervisor and department
Greer, John (Physiology)
Thébaud, Bernard (Physiology)
Examining committee member and department
MacLean, Joanna (Pediatrics)
Alexander, Todd (Physiology)
Greer, John (Physiology)
Thébaud, Bernard (Physiology)
Department of Physiology

Date accepted
Graduation date
Master of Science
Degree level
Bronchopulmonary dysplasia (BPD) is a common complication of extreme prematurity. Preterm infants often require mechanical ventilation and supplemental oxygen for survival. These interventions increase the risk of developing BPD. Despite its frequency, there remains no effective treatment for BPD. Evidence suggests that mesenchymal stromal cells (MSCs) prevent oxygen-induced lung injury in rodent models of BPD. The benefits observed appear mediated via paracrine effects. We explored the ability of cell-free conditioned media (CDM) from human umbilical cord-derived MSCs (MSC-CDM) to prevent lung damage in a neonatal mouse model of BPD combining inflammation, ventilation-induced lung injury, and supplemental oxygen. Neonatal mice (C57BL/6) were mechanically ventilated at postnatal day 9-10 for 8 hours with a tidal volume of 10µl/g, 180 breaths/minute and 40% oxygen. Inflammation was induced by intraperitoneal administration of lipopolysaccharide (LPS) 48 hours preceding ventilation. Age matched unventilated mice that did not receive LPS were used as controls. The treatment group received intratracheal MSC-CDM (3µl/g) immediately prior to mechanical ventilation. At completion of ventilation lungs were harvested for structural and molecular analysis. Ventilated mice that received LPS exhibited alveolar simplification and reduced vascular density compared to controls as demonstrated by a significantly greater mean linear intercept and reduced number of vessels per high power field (p<0.05). These mice also exhibited elevated levels of macrophage inflammatory protein – 2 (MIP-2) and monocyte chemoattractant protein – 2 (MCP-1) compared with controls (p<0.05). Intratracheal MSC-CDM treatment significantly attenuated structural lung injury and improved vascular density compared with untreated, ventilated mice (p<0.05), but did not significantly reduce levels of MIP-2/MCP-1. Treatment with MSC-CDM attenuates structural lung injury and improves vessel density in a clinically relevant neonatal mouse model of BPD. By harnessing the beneficial effects of MSC-CDM, new therapies for treating BPD may be developed.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Strueby L, Thébaud B. Advances in bronchopulmonary dysplasia. Expert Rev Respir Med. 2014;8(3): 327-338

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 6366820
Last modified: 2015:10:22 06:07:47-06:00
Filename: Strueby_Lannae_L_201411_MSc.pdf
Original checksum: e2ac47d4f920bc8e28c7e00ae6bf2eb6
Activity of users you follow
User Activity Date