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Al-Induced, 51-Kilodalton, Membrane-Bound Proteins Are Associated with Resistance to Al in a Segregating Population of Wheat Open Access


Author or creator
Taylor, G. J.
Basu, A.
Slaski, J. J.
Zhang, G.
Good, A.
Additional contributors
Barley roots
Translocating inorganic pyrophosphatase
Nutrient solutions
Aluminum tolerance
Triticum-Aestivum L
Type of item
Journal Article (Published)
Incorporation of S-35 into protein is reduced by exposure to Al in wheat (Triticum aestivum), but the effects are genotype-specific. Exposure to 10 to 75 mu M Al had little effect on S-35 incorporation into total protein, nuclear and mitochondrial protein, microsomal protein, and cytosolic protein in the Al-resistant cultivar PT741. In contrast, 10 mu M Al reduced incorporation by 21 to 38% in the Al-sensitive cultivar Katepwa, with effects becoming more pronounced (31-62%) as concentrations of Al increased. We previously reported that a pair of 51-kD membrane-hound proteins accumulated in root tips of PT741 under conditions of Al stress. We now report that the 51-kD band is labeled with S-35 after 24 h of exposure to 75 mu M Al. The specific induction of the 51-kD band in PT741 suggested a potential role of one or both of these proteins in mediating resistance to Al. Therefore, we analyzed their expression in single plants from an F-2, population arising from a cross between the PT741 and Katepwa cultivars. Accumulation of 1,3-beta-glucans (callose) in root tips after 24 h of exposure to 100 mu M Al indicated that this population segregated for Al resistance in about a 3:1 ratio. A close correlation between resistance to Al (low callose content of root tips) and accumulation of the 51-kD band was observed, indicating that at least one of these proteins cosegregates with the Al-resistance phenotype. As a first step in identifying a possible function, we have demonstrated that the 51-kD band is most clearly associated with the tonoplast. Whereas Al has been reported to stimulate the activity of the tonoplast HC-ATPase and H+-PPase, antibodies raised against these proteins did not cross-react with the 51-kD band. Efforts are now under way to purify this protein from tonoplast-enriched fractions.
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© 1997 American Society of Plant Biologists. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
Citation for previous publication
Taylor, G.J., Basu, A., Slaski, J.J. Zhang, G. & Good, A. (1997). Al-Induced, 51-Kilodalton, Membrane-Bound Proteins Are Associated with Resistance to Al in a Segregating Population of Wheat. Plant Physiology, 114(1), 363-372.
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