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Permanent link (DOI): https://doi.org/10.7939/R34T3H

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Melano-macrophage characterization and their possible role in the goldfish (Carassius auratus) antibody affinity maturation Open Access

Descriptions

Other title
Subject/Keyword
Melano-macrophage
Affinity maturation
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Diaz Satizabal, Laura P
Supervisor and department
Magor, Brad G (Biological Sciences)
Examining committee member and department
Belosevic, Miodrag (Biological Sciences. School of Public Health)
Barreda, Daniel (Biological Sciences. Agricultural, Food & Nutritional Science)
Hanington, Patrick (School of Public Health)
Department
Department of Biological Sciences
Specialization
Physiology, Cell and Developmental Biology
Date accepted
2013-04-02T14:53:38Z
Graduation date
2013-06
Degree
Master of Science
Degree level
Master's
Abstract
During the antibody affinity maturation process, changes to the immunoglobulin genes are initiated by the B-cell specific enzyme activation-induced cytidine deaminase (AID). These changes are randomly generated and produce antibodies with different affinities for the antigen. In mammals, this process occurs in highly regulated environments known as germinal centres (GCs). Although conventional GCs are absent in fish, AID expressing B-cells are found in close proximity to pigmented myeloid cells called melano-macrophages (MMs). Previous in vivo work suggested that MMs have functions consistent with those of follicular dendritic cells (trapping antigen) and tingible body macrophages (phagocytosis of apoptotic cells) found in the GC. Quantitative PCR used to assess gene expression of pro- and anti-inflammatory cytokines in MMs under in vivo and in vitro conditions revealed that these cells may have different roles in the immune response depending on their anatomical location.
Language
English
DOI
doi:10.7939/R34T3H
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File author: Laura Satizabal
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