ERA

Download the full-sized PDF of Development of Rosette Nanotubes For HIV-1 TherapyDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3JH3D980

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Development of Rosette Nanotubes For HIV-1 Therapy Open Access

Descriptions

Other title
Subject/Keyword
HIV-1 Therapy
Peptide synthesis
Rosette Nanotubes
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Sharma, Kumakshi
Supervisor and department
Dr. Robert E. Campbell (Department of Chemistry)
Dr. Hicham Fenniri (Department of Chemistry)
Examining committee member and department
Dr. Hicham Fenniri (Department of Chemistry)
Dr. Robert E. Campbell (Department of Chemistry)
Dr. Nils O. Petersen (Department of Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2014-09-24T14:58:22Z
Graduation date
2014-11
Degree
Master of Science
Degree level
Master's
Abstract
The development of therapeutics to target dendritic cells (DCs) for the treatment of diseases is of great importance, as these cells are known to play an important role in antigen processing and presentation in the immune system. Herein, we explore the applications of biocompatible, self-assembled rosette nanotubes (RNTs) as a therapeutic agent to deliver a HIV antigen to the DCs. ELDKWA is linear fragment (epitope) present on the ectodomain of HIV-1 envelope glycoprotein 41 complex, which recognizes the galactoceramide receptors on the DC cell surface and lysine (K) acts as a spacer between the peptide ELDKWA and the RNTs. Our strategy is to functionalize the RNTs on the outer surface with the bio-active peptides ELDKWAK, RGDSK and DRGSK. RGD is a cell-adhesion peptide, which may aid in the attachment of the HIV-1 antigen (ELDKWA) to the DC cell surface and DRG is the scrambled version of RGD. The RNTs with the bioactive peptides were co-mixed with fluorescently-labeled RNTs to study the cell uptake of the RNTs.
Language
English
DOI
doi:10.7939/R3JH3D980
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-09-24T20:58:34.013+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 9779292
Last modified: 2016:06:24 17:42:58-06:00
Filename: Sharma_Kumakshi_201409_MSc.pdf
Original checksum: e260c2c7389199c410fac927e8d21075
Activity of users you follow
User Activity Date