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Permanent link (DOI): https://doi.org/10.7939/R3F981

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Molecular regulation and endogenous expression of CRTh2 in in vitro differentiated CRTh2+ Th2 cells Open Access

Descriptions

Other title
Subject/Keyword
prostaglandin D2
CRTh2
Th2 cells
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
MacLean, Emily Iris
Supervisor and department
Cameron, Lisa (Medicine)
Vliagoftis, Harissios (Medicine)
Examining committee member and department
Burshtyn, Deborah (Medical Microbiology and Immunology)
Baldwin, Troy (Medical Microbiology and Immunology)
Department
Medicine
Specialization

Date accepted
2011-07-19T14:59:31Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
Allergic inflammation is mediated by T helper 2 (Th2) cells. Chemoattractant receptor homologous molecule expressed on Th2 cells (CRTh2) is expressed by Th2 cells and mediates chemotaxis and production of the Th2 cytokines interleukin (IL)-4, IL-5 and IL-13. To understand the molecular regulation of CRTh2, we studied the promoter and found putative binding sites for GATA3 and nuclear factor in activated T cells (NFAT), transcription factors known to regulate Th2 cytokine expression. We hypothesized these factors also regulate transcription of CRTh2. Using a reporter construct with the proximal region of the CRTh2 promoter, we found transcription of CRTh2 was increased following stimulation in CRTh2+ Th2 cells. Endogenous CRTh2 was decreased following stimulation. Further, over-expression of NFAT1 decreased a GATA3 dependent increase in CRTh2 promoter activity. The discrepancy between CRTh2 endogenous expression and proximal promoter activity indicates longer regions of the CRTh2 5’ regulatory region may be important for regulation.
Language
English
DOI
doi:10.7939/R3F981
Rights
License granted by Emily MacLean (emaclean@ualberta.ca) on 2011-07-14T23:15:15Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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